Fig. 3

Y-2 reduces astrogliosis and microgliosis in AD mice. Images show the IHC staining of GFAP (green) (A) and Iba1 (green) (C) in cortex and hippocampus of the 5×FAD mice. Nuclei counterstained with DAPI (blue). Quantification shows the average number per mm² of GFAP-positive astrocytic clusters with indicated sizes (diameter < 20 μm, 20–40 μm and > 40 μm) (B) and Iba1-positive microglial clusters (D). n = 40, n represents the number of randomly selected slices from 5 mice in each group. one-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001. All data are represented as mean ± SD. Scale bar, 100 μm