Fig. 8

Glucose and CUMS inhibited IκBα expression and facilitated NF-κB p65 nuclear translocation via GLUT1. (A) Levels of IκBα proteins in BV2-LV-GFP/LV-GLUT1 cells treated with or without glucose were determined by western blotting, and the quantified result is shown (n = 3, One-way ANOVA with Tukey’s post hoc test). (B) NF-κB p65 in the cytosolic/nuclear fractions of BV2-LV-GFP/LV-GLUT1 cells treated with or without glucose was determined by western blotting. The quantified result is shown (n = 3, One-way ANOVA with Tukey’s post hoc test). (C) NF-κB nuclear translocation in BV2-LV-GFP/LV-GLUT1 cells under control or glucose conditions was analyzed by IF staining. Representative images are shown. Scale bar, 20 μm. (D) Levels of IκBα in BV2 cells exposed to STF-31 or BAY-876 upon glucose treatment were determined by western blotting. The quantified result is shown (n = 3, One-way ANOVA with Tukey’s post hoc test). (E) NF-κB p65 in the cytosolic/nuclear fractions of BV2 cells exposed to STF-31 or BAY-876 upon glucose treatment was determined by western blotting. The quantified result is shown (n = 3, One-way ANOVA with Tukey’s post hoc test). (F) NF-κB nuclear translocation in BV2 cells exposed to STF-31 or BAY-876 under glucose incubation was analyzed by IF staining. Representative images are shown. Scale bar, 20 μm. (G&H) Levels of IκBα (G), and NF-κB p65 in cell cytosolic/nuclear fractions (H) in hippocampus lysates from CTRL and CUMS mice with AAV-mirGLUT1 or AAV-control injection were determined by western blotting. The quantified result is shown (n = 3, One-way ANOVA with Tukey’s post hoc test). *p < 0.05, **p < 0.01