Skip to main content
Fig. 5 | Cell & Bioscience

Fig. 5

From: GLUT1-mediated microglial proinflammatory activation contributes to the development of stress-induced spatial learning and memory dysfunction in mice

Fig. 5

CUMS and glucose upregulate GLUT1 expression in microglia. (A) Schematic of microglia isolated from the hippocampus of CTRL and CUMS mice. (B) qRT-PCR assays validating the expression of biomarkers in isolated microglia versus non-microglia cells (n = 4, Student’s t-test). Cx3cr1, microglial biomarker; Snap25, neuronal biomarker; GFAP, astrocyte biomarker. (C) qRT-PCR assays monitoring the expression of GLUTs in derived microglia from CTRL and CUMS hippocampal tissues (n = 4, Student’s t-test). N.D, not detected. (D) qRT-PCR assays monitoring the expression of GLUTs in glucose-treated BV2 cells and control cells (n = 4, Student’s t-test). N.D, not detected. (E) Western blot of the expression of GLUT1 protein in glucose-treated BV2 cells and control cells. (F) Representative images of GLUT1 staining in glucose-treated BV2 cells and control cells. Scale bar, 50 μm. **p < 0.01

Back to article page

Cell & Bioscience

ISSN: 2045-3701

Contact us