Fig. 1

Establishment of patient-derived PA and CXPA organoids. (a) Schematic overview of the tumour-derived organoid culture. (b) Bright-field images of PA and CXPA organoids in the first generation on days 7, 11, and 14. Scale bars = 100 µm. (c) Overview of PA and CXPA organoids under bright-field and H&E staining results of PA and CXPA organoids and their parental tumours. Scale bars = 100 µm. (d) Bright-field images of PA (generations 1, 2, and 3) and CXPA (generations 1, 3, and 5) organoids. (e) Representative image of PSR-stained positive areas (in red colour, red arrowhead) of CXPA organoids and their parental tumours. (f) Representative image of PSR-stained positive areas (in red colour, black arrowhead) of PA organoids and their parental tumours. Scale bars = 100 µm