Fig. 4

Calcium and peristalsis-related protein localization and gene expression in Ahnak KO mouse kidney at PN1. (A) Best3 is broadly observed in WT glomerulus. Best3 is localized in glomerulus and proximal tubules. (E) Best3 is weakly detected in Ahnak KO kidney compared with WT kidney. (B) Cacnb4 is strongly observed in glomerulus and surrounding tubules. (F) Cacnb4 is faintly detected in Ahnak KO kidney compared to WT. (C) P2rx3 is broadly and strongly localized in WT glomerulus and surrounding tubules. (G) P2rx3 is weakly observed in Ahnak KO kidney compared to WT. (D) Peristalsis marker c-kit is localized in basement membrane of WT kidney pelvis. (H) c-kit is not detected in Ahnak KO kidney pelvis. (I) Expression level of Best3 is reduced in Ahnak KO kidney compared with WT at PN1. (J) Cacnb4 expression level is down-regulated in Ahnak KO compared to WT. (K) Expression level of P2rx3 is decreased in Ahnak KO compared with WT kidney at PN1. (L) Expression level of c-kit is reduced in Ahnak KO kidney. (M) Expression level of α-SMA is not altered in Ahnak KO kidney at PN1. (N) Best3 protein level was reduced in Ahnak KO mouse Kidney at PN1. White dotted line, glomerulus; **, P < 0.01; ***, P < 0.001; ns, not significant, Scale bar; A-H 100 μm