Fig. 2

Phenotypic comparison between WT and Ahnak KO kidney and ureter. (A-H) At E15.5 and PN1, hydronephrosis with expanded vacant region (asterisks) is observed in Ahnak KO kidney compared to WT. (I-P) Well developed tunica mucosa, tunica muscularis and tunica adventitia are observed in WT and Ahnak KO distal ureter. Abnormal tunica mucosa, tunica muscularis and tunica adventitia formation is detected in Ahnak KO proximal ureter. (Q) Kidney width is not significantly different between WT and Ahnak KO mice. (R) Hydronephrosis ratio is increased approximately 8-fold in Ahnak KO kidney. (S) Urothelium thickness is significantly reduced in Ahnak KO ureter compared with WT. (T) Hydroureter ratio is dramatically increased in Ahnak KO ureter. (U, V) Relative lumen diameter per 200 μm position from proximal to distal region shows that hydroureter presents proximal region in Ahnak KO at PN1. Scale bar; A, B, C, D, 100 μm; E, F, G, H, 500 μm; I, J, M, N, 100 μm; K, L, O, P 50 μm