Fig. 1

Localization of Ahnak in developing WT kidney and ureter. (A-D) Ahnak is localized in ureteric bud (UB) at E12.5 WT kidney. K8 marks collecting duct, co-localized with Ahnak in the collecting duct cell membrane. (D) High magnification image showing that Ahnak is strongly localized in the UB but weakly in the metanephric mesenchyme (MM). (E) At E15.5 WT kidney, Ahnak is detected in collecting duct and glomerulus. Renal mesenchyme has weak Ahnak localization. (F) Ahnak is localized in collecting duct membranes with K8. (G) In glomerulus, Ahnak is detected in podocytes. (H) Double staining results shows that Ahnak is not co-localized with lotus tetragonolobus lectin (LTL), a proximal tubule marker. (I-L). The overall localization tendency of Ahnak at PN1 is similar to E15.5. (M-R) Ahnak and E-cadherin are co-localized in tunica mucosa. Ahnak is detected in tunica muscularis and tunica adventitia during ureter development. UB; Ureteric bud, MM; Metanephric mesenchyme, Tm; Tunica mucosa TM; Tunica muscularis TA; Tunica adventitia. Scale bar; A, B, C, G, K, N, P, R, 100 μm; D, 150 μm; E, 250 μm; F, J, 40 μm; H, M, O, Q, 100 μm; I, 500 μm; L, 200 μm