Fig. 6

Zebrafish brain structural/cellular phenotyping with GFAP staining. A and B Three-dimensional view of the whole brain (A) and telencephalon (B) with GFAP staining in juvenile zebrafish. The shape and precise location of astrocytes in the CNS were displayed in brain-wide perspective. (Voxel size: 1.25 μm × 1.25 μm × 3 μm for A; Voxel size: 0.624 μm × 0.624 μm × 3 μm for B). C Three-dimensional view of the whole brain with GFAP staining in adult zebrafish. The shape and precise location of astrocytes in the CNS were displayed in brain-wide perspective. (Voxel size: 1.25 μm × 1.25 μm × 5 μm). D Three-dimensional view of the whole brain with GFAP and HuC/D staining in juvenile zebrafish. GFAP-positive cells are located in most regions of the brain, running radially from the ventricle to the pial surface (Voxel size: 1.25 μm × 1.25 μm × 3 μm; green, GFAP; red, HuC/D). E–G Images of neuron and astrocytes with HuC/D and GFAP staining in juvenile zebrafish. Astrocytes showed long processes radiating from the cell body and the terminal expansions of cytoplasmic processes called end feet. Astrocytes are located among neurons and interact with several neighbor neurons. (Voxel size: 0.312 μm × 0.312 μm × 2 μm for E and F; Voxel size: 0.078 μm × 0.078 μm × 2 μm for G; green, GFAP; red, HuC/D)