Table 1 Cryo-EM data statistics
| Â | Human PRPS2-short filament |
EM equipment | Titan Krios |
Detector | K3 camera |
Magnification | 22,500x |
Voltage (kV) | 300 |
Electron exposure ((e–/Å2)) | 60 |
Defocus range(μm) | − 1.0 to − 2.5 |
Pixel size(Ã…) | 1.06 |
Symmetry imposed | D3 |
Number of collected movies | 2403 |
Initial particle images (no.) | 681,672 |
Final particle images (no.) | 140,303 |
Refinement | |
 EMDB ID | EMD-33883 |
 PDB code | 7YK1 |
 Initial model used (PDB code) | - |
 Map resolution (Å) | 3.1 |
  FSC threshold | 0.143 |
 Map resolution range (Å) | 2.9–3.9 |
 Map sharpening B-factor(Å2) | -74 |
Model composition | |
 Non-hydrogen atoms | 14,364 |
 Protein residues | 1836 |
 Ligands | ADP,Pi,Mg |
 Ions | 12 |
B factors(Ã…2) | |
 Protein | 58 |
 Ligand | 67 |
R.m.s. deviations | |
 Bond lengths (Å) | 0.005 |
 Bond angles (°) | 0.690 |
Validation | |
 MolProbity score | 1.70 |
 Clashscore | 5.53 |
 Poor rotamers (%) | 0.13 |
Ramachandran plot | |
 Favored (%) | 93.98 |
 Allowed (%) | 5.24 |
 Disallowed (%) | 0.77 |