Fig. 3
Contact of hexamer in human PRPS2 filament. A and B Maps and models of human PRPS2 filament between adjacent hexamers. The residues responsible for the interactions are indicated. The area marked in red is that shown in Figure B. C Negative staining of human PRPS filament. The wild-type human PRPS2 can form filament under ADP + Mg2+, ATP + R5P + Pi + Mg2+,or Pi + PRPP + Mg2+ condition. Mutant R301A and R302A can disrupt filament formation. The scale bar is 200 nm. D Enzyme activity assay of wild-type human PRPS2 and its mutant. Mutant R301A and R302A almost lost its activity