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Fig. 5 | Cell & Bioscience

Fig. 5

From: Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice

Fig. 5

In vivo and in vitro OT activity analysis. (A) BEAT quantification of the bystander C4 and C5 editing in mice treated with or without AAV9. ****p < 0.0001; two-tailed unpaired t test. (B, C) Targeted deep sequencing of the genomic DNA PCR amplicons of the OT1 (B) and OT2 (C) from N2a cells transfected with or without Angptl3-gRNA1 combined with N- and C-terminal halves of AncBE4max. *p < 0.05; **p < 0.01; ***p < 0.001; two-tailed unpaired t test. (D) Targeted deep sequencing to quantify the gRNA-independent off-target editing using orthogonal R-loop assay. *p < 0.05; **p < 0.01; ***p < 0.001; two-tailed unpaired t test

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Cell & Bioscience

ISSN: 2045-3701

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