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Fig. 1 | Cell & Bioscience

Fig. 1

From: Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice

Fig. 1

In vitro validation of Angptl3 gRNAs to introduce a premature stop codon with CBE. (A) Schematic showing two overlapping Angptl3 gRNAs (gRNA1 and gRNA2) with different PAM-targeting CBEs (AncBE4max or CBE-SpG). The targeted cytosine is colored in red and the PAM sequences are colored in blue. (B) Comparison of the editing efficiency of gRNA1 and gRNA2 at mouse Angptl3 locus. ****p < 0.0001, ***p < 0.001; one-way ANOVA with Turkey’s multiple comparisons test. (C) Measurement of base editing efficiency induced by Angptl3- gRNA1 when combined with N- and C-terminal halves of AncBE4max. ****p < 0.001; two-tailed unpaired t test

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Cell & Bioscience

ISSN: 2045-3701

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