Fig. 8
From: Spatiotemporal control of genome engineering in cone photoreceptors
Intact retinal function in Arr3P2ACreERT2 driver. A At PD40, mice with KI alleles had larger scotopic a- wave amplitudes only at 1.0 log cd.s/m2 intensity; F(3,21) = 3.414, p = 0.036 (One-way ANOVA, Additional file 1: Table S5) (A, left upper). There was no significant difference in scotopic b- wave and photopic a- wave amplitudes between mice of four different genotypes (A, left lower, right upper). Male hemizygous and female homozygous mice had smaller photopic b- wave amplitudes compared to the b- wave amplitudes of heterozygous and wild-type mice at −1.0, 0, 0.3, 0.6, and 1.0 log cd.s/m2 intensities (One-way ANOVA, Additional file 1: Table S5) (A, right lower). B At PD210, female homozygous mice had large photopic b- wave amplitudes at 1.48, 2.0 and 2.3 log cd.s/m2 intensities (One-way ANOVA, Additional file 1 Table S6). There was no difference in scotopic a-, b-, and photopic a- wave amplitudes in mice of three different genotypes (B, upper). (C) Representative flicker series recordings from mice of different genotypes at PD40 an PD210. (D–E) Male hemizygous and female homozygous mice tended to have larger amplitudes at low stimulus frequencies and lower amplitudes at higher stimulus frequencies than wild-type mice. These differences are significant in younger mice (PD40) at 5, 7, 10, 12, 15, 18, 20 and 30 Hz frequencies (D) and in older mice (P210) at 0.5, 20 and 30 Hz frequencies (E) (One-way ANOVA, Additional Table S7). Data represent mean ± 2SE. For PD40, N = 7, 3, 7, and 8 mice for WT, female heterozygous Arr3P2ACreERT2/+, male hemizygous and female homozygous Arr3P2ACreERT2 mice, respectively. For PD210, N = 11, 6, and 6 mice for C57BL/6 J, male hemizygous and female homozygous Arr3P2ACreERT2 mice, respectively. *: p < 0.05; **: p < 0.01; ***: p < 0.001