Fig. 7
From: Spatiotemporal control of genome engineering in cone photoreceptors
Intact retina/cone structure in Arr3P2ACreERT2 driver. A Horizontal and vertical retinal OCT images from representative 2 month-old WT, female heterozygous Arr3P2ACreERT2/+, male hemizygous and female homozygous Arr3P2ACreERT2 mice. B Quantification of the total retinal and outer nuclear layer thickness showed no significant difference between the four groups (One-way ANOVA, Additional file 1: Table S4). Data represent mean ± 2SE. N = 5, 5, 6, and 4 mice for WT, female heterozygous, male hemizygous, and female homozygous Arr3P2ACreERT2 mice, respectively. The mean total retinal thicknesses were 225.86, 224.05, 228.70, and 227.15 μm, respectively. The mean outer nuclear layer thicknesses were 60.92, 60.03, 59.77, and 59.28 μm, respectively. C–D Representative retinal sections from a PD322 WT and a PD318 male hemizygous Arr3P2ACreERT2 mouse were stained with cone markers (antibodies against M-opsin and S-opsin). Fewer M-opsin positive (red) and S-opsin positive (green) cones were present in the dorsal retinal compared to cones in the ventral retinal in both WT and Arr3P2ACreERT2 mice. ONL outer nuclear layer, OS outer segment, ns not statistically significant. Scale bars, 200 μm in A, and 20 μm in C and D