Fig. 6
From: Spatiotemporal control of genome engineering in cone photoreceptors
Comparable ARR3 protein expression in Arr3P2ACreERT2 mice and B6 controls. A Immunoblot of retinal lysates collected from mice at PD55 involved in cone phototransduction shows comparable protein expression in WT, heterozygous, and homozygous Arr3P2ACreERT2 mice, quantified by ARR3, M-opsin, and S-opsin from left to right. F(2, 6) = 0.756, p = 0.510; F(2,6) = 1.514, p = 0.294; F(2, 6) = 1.109, p = 0.389, respectively (One-way ANOVA). N = 3 mice for each group. B Immunoblot of proteins from mice at PD230 involved in cone phototransduction shows comparable protein expression in WT and homozygous Arr3P2ACreERT2 mice, quantified by ARR3, M-opsin, and S-opsin from left to right, p = 0.919, 0.764, 0.688, respectively. N = 3 mice for each group. C qPCR analysis of gene expression for Arr3, Cnga3, Opn1mw in WT and homozygous at PD55 (left), p = 0.057, 0.057, 0.857, respectively; and PD230 (right), p = 0.700, 0.400, 0.400, respectively. N = 4 for WT and homozygous group at PD55. N = 3 for WT and homozygous groups at PD230. Black dots indicate WT, green dots indicate heterozygous, and red dots indicate homozygous Arr3P2ACreERT2 mice. Data represent mean ± 2SE in A, B, and C. *: p < 0.05; **: p < 0.01; ***: p < 0.001