Fig. 5
From: Spatiotemporal control of genome engineering in cone photoreceptors
Cre-LoxP recombination efficiency was 100% in hemizygous Arr3P2ACreERT2 driver. A Noninvasive in vivo fundus short-wavelength autofluorescence (SW-AF) showed homogenously increased intensity in male hemizygous Arr3P2ACreERT2Ai14D+/- compared to the SW-AF in WT (C57BL/6 J) mice. In female heterozygous Arr3P2ACreERT2/+Ai14D+/- mice, there was a mosaic pattern of SW-AF images when tamoxifen induction was delivered at PD21-24 or PD2-4 (upper right and lower right). B Immunohistochemistry (IHC) staining on a retinal whole mount and crysections from male hemizygous Arr3P2ACreERT2Ai14D+/- mice showed distinct tdTomato expression in cone cells with ARR3 barely labeled in green (1st and 2nd rows; Additional file 1: Movie S6, S7). When the same sections were labeled for another cone cell marker, glycogen phosphorylase (GlyPh), we found 100% colocalization of GlyPh (green) and tdTomato (red) (bottom; Additional file 1: Movie S8), suggesting that the Cre-LoxP recombination efficiency was 100% in hemizygous Arr3P2ACreERT2Ai14D+/- mice. C–D IHC staining for the anti-ARR3 antibody on a retinal whole mount and cryosections from a female heterozygous Arr3P2ACreERT2/+Ai14D+/- mouse showed a mosaic pattern of green (ARR3-positive cone cells) and red (tdTomato expressing cone cells) in retinal whole mounts and cryosections (1st and 2nd rows; Additional file 1: Movies S9, S10). Scale bars, 200 μm in A, 20 μm in B and D, and 1000 μm in C