Fig. 4
From: Spatiotemporal control of genome engineering in cone photoreceptors
Impact of KI T2A-CreERT2 cassette on retinal protein and function in Arr3T2ACreERT2 driver. A Immunoblot of retina lysates from mice at PD60 using anti-ARR3 and anti-M-opsin antibodies revealed comparable protein expression in WT, heterozygous, and homozygous Arr3T2ACreERT2 mice quantified by ARR3 and M-opsin on the right. F(2, 6) = 0.616, p = 0.570; F(2,6) = 0.964, p = 0.433, respectively (One-way ANOVA). N = 2, 3, and 4 mice for WT, female heterozygous, and female homozygous Arr3T2ACreERT2 mice, respectively. B Scotopic serial intensity ERG showed no significant difference in a- or b- wave amplitudes between mice of three different genotypes. (One-way ANOVA, Additional file 1: Table S3). N = 4, 4, and 7 mice for WT, female heterozygous, and female homozygous Arr3T2ACreERT2 mice, respectively. C Histology showed normal retinal thickness and outer nuclear layers at PD385 homozygous Arr3T2ACreERT2 and age-matched WT mice. Data represent mean ± 2SE in A and B