Fig. 2
From: Spatiotemporal control of genome engineering in cone photoreceptors
Generation of Arr32ACreERT2 driver. A KI of a 2A peptide with the CreERT2 sequence to the stop codon “TAG” of Arr3 gene in exon 17 in mouse embryonic stem (ES) cells; confirmation of germline transmissions by crossing the male chimera with female ACTB-FLPe mice to remove the neomycin cassette; genotyping for the KI allele by primers F1 and R1 and for the wild type (WT) allele by the primers F1 and R2. B Electrophoresis of the amplicons of the KI allele (~ 254 bp) and the WT allele (~ 437 bp). C Schematic representation of assessing recombination specificity and efficiency of the Cre-LoxP: a female homozygous Arr32ACreERT2 mouse was crossed with a male Ai14D reporter mouse to generate male hemizygous Arr32ACreERT2Ai14D+/- and female heterozygous Arr32ACreERT2/+Ai14D+/- mice