Fig. 2
From: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Isg15 deletion promotes follicular development in mice. A The representative ovarian morphology. Ovaries in the representative images were collected from three-week-old and eight-week-old Isg15+/+ and Isg15−/− mice. The below is the ratio of ovarian weight to body weight (n = 6). B, C H&E staining of ovarian sections from three-week-old (B) and eight-week-old (C) Isg15+/+ and Isg15−/− mice. The rightmost panel is the number of follicles at different development stages in three-week-old and eight-week-old mice. CL: corpora lutea. Scale bar: 200 μm. D, E Quantitative RT-PCR (D) analysis and western blot (E) analysis of ovulation-related genes including Adamts1, Ptgs2, Has2, Areg, Ereg in eight-week-old Isg15+/+ and Isg15−/− ovaries. The right bar graph showed the quantification of protein level. All data are mean ± SD; *P < 0.05, **P < 0.01