Fig. 5

LSD1 interacts with core members of PRC2 and plays a significant role in the artificial H3K27me3-H3K4me1 transition in mESCs. A Scatterplot showing the positive correlation of expression between LSD1 and EED. Significance was examined with paired t-test, p value = 0, R = 0.79. B The STRING website predicts interaction between LSD1 and SUZ12, EED and EZH2. C Left panel: mESC cell nuclear extract was immunoprecipitated by an anti-LSD1 antibody, and subjected to western blotting analysis with anti-EED and anti-SUZ12 antibody; Right panel: mESC cell nuclear extract was immunoprecipitated by an anti-EED antibody, and subjected to western blotting analysis with anti-LSD1 and anti-SUZ12 antibody. D Heatmaps and average line plots showing LSD1 patterns at promoter CGIs and their shores of up-regulated (Up) and down-regulated (Down) genes in bimodal-loss group in WT and Eed^−/− mESCs. OG2: WT OG2 mESCs; OG2 Eed^−/−: Eed^−/− OG2 mESCs. E Boxplot showing the differences of normalized LSD1 coverage at bivalent promoter CGIs between WT and Eed^−/− OG2 mESCs of up-regulated (Up) and down-regulated (Down) genes in bimodal-loss group. Significance was examined t-test, ****p value < 0.0001. F IGV browser track showing histone modifications patterns and the level of LSD1 chromatin binding at promoter CGIs of neurodevelopmental bivalent genes in WT and Eed^−/− OG2 mESCs