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Table 1 Primary and secondary antibodies for Western Blotting

From: A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer’s disease exacerbated with high fat diet

Protein

Antibody

ADAM10

ab124695 (abcam)

App

SIG-39152 (Convance)

App C terminal fragment

SIG-39152 (Convance)

DBN1

ABN 207 (Merck Millipore)

GAPDH

MAB374 (Merck Millipore)

GSK3β

#9315 (Cell Signaling Technology)

P-GSK3β (TYR216)

ab75745 (abcam)

IDE

ab32216 (abcam)

IRS2

4502S (Cell Signaling)

Neurexin

ab34245 (abcam)

PTP1B

GTX55767 (Genetex)

sAPPβ

SIG-39138-0 (Covance)

Synaptophisin

M0776 (Dako)

Tau

GTX112981 (Genetex)

P-Tau(ser396)

44752G (Invitrogen)

P-Tau(ser404)

44-758G(Invitrogen)

TLR4

Sc-293072 (Santa Cruz Biotechnology)

Β-actin

A5441 (Sigma)

2nd-ary Goat anti-Rabbit

31460 (Invitrogen)

2nd-ary Goat anti-Mouse

31430 (Invitrogen)