Fig. 8

Modifications in membrane and vesicular transport proteins upon HBV integration. a, b, e L/H ratios for selected regulated Kub-sites & proteins from three biological replicates in HepG2.2.15 cells. c Co-immunoprecipitation and immunoblot analysis of extracts of Huh7 cells transfected with various combinations of plasmid encoding FLAG-tagged SLC1A4, SLC1A5 and Hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) and treated with MG132, are shown. d The levels of the secreted HBeAg and HBsAg were determined by ELISA from the cell culture supernatants samples co-transfected (1:1) with pHBV1.3 and flag-tagged SLC1A4 and SLC1A5, are shown. f Co-immunoprecipitation and immunoblot analysis of extracts of Huh7 cells transfected with plasmid encoding FLAG tagged-HGS and Hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) and treated with MG132, are shown. g, h Co-immunoprecipitation and immunoblot analysis of extracts of Huh7 cells transfected with FLAG-tagged VAMP8 and HA-tagged wild type ubiquitin, Hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) and treated with MG132, are shown. The levels of the secreted HBeAg and HBsAg were determined by ELISA from the cell culture supernatants samples co-transfected (1:1) with pHBV1.3 and flag-tagged VAMP8 and its K-47-R lysine to Arginine mutant. i Co-immunoprecipitation and immunoblot analysis of extracts of Huh7 cells transfected with FLAG-tagged RAB8A and Hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) and treated with MG132, are shown