Fig. 4

RRM2 silencing induced DNA damage in LUAD cells. a, b GSEA for the enriched gene sets in the RRM2 high- and low-expression groups. c, d immunofluorescence was performed to detect γH2AX foci formation. The representative images were taken with confocal microscope. Scale bar: 10 μm. e, f Comet assay was performed after transfecting siRRM2 into A549 and PC9 cells for 48 h. Representative pictures were shown. Scale bar: 50 μm. The percentage of tail DNA content and tail length of the comet was quantified and graphed. g Immunoblotting of BRCA1, P53 and γH2AX. GAPDH was used as an internal control. h Relative intensity of γH2AX in A549 and PC9 cells after RRM2 knockdown. All tests were repeated 3 times. *p < 0.05, **p < 0.01