Fig. 3
MSCs alleviate docetaxel-induced CRPC cells proliferation decrease and apoptosis increase in vitro. a PC3 cells (2 × 105/well) were cocultured with MSCs in a 6-well plate or the conditioned medium collected from MSCs were added in PC3 culture medium with an existence of DTX (20 μM) for 48 h. CCK8 assay was used to examined the cell viability of PC3 cells. b The cell apoptosis of PC3 cells was analyzed by flow cytometry using Annexin V-FITC/PI. c CCK8 assay was used to examined the cell viability of DU145 cells. d The cell apoptosis of DU145 cells was analyzed by flow cytometry using Annexin V-FITC/PI. *P < 0.05; **P < 0.01