Fig. 3
The in vivo protective function of HSP20-abundance against oxidative damages. Mice were repeatedly exposed to 0.5 ppm O3 (preconditioned, n = 10) or filtered air (FA) (control, n = 10) for 1 h on every other day for 7 days. Forty-eight hours after the last exposure, equal number (n = 5) of preconditioned or control mice were stimulated with 2.0 ppm O3 or FA for 3 h, and BAL fluid samples and lung tissues were harvested 24 h after the stimulation. a Mice bodyweight before and after the high-level-O3 stimulation. b Total and differential cell counts in BAL fluid samples. c Typical pictures of H&E staining on lung sections (Black bar represents 40 μm, red arrow indicating inflammatory cells, b = bronchi, v = vessel). d Histological inflammation in perivascular (PV), peribronchial (PB) area and their average. e Airway epithelial cell density on basal lamina of distant bronchi on H&E stained lung sections. f, g The concentration of Albumin and E-cadherin in cell-free BAL fluid. h, i The content of MDA and the activity of GSH-Px in lung tissue homogenization. j The mRNA expression of NQO-1 in lung tissues. Data are expressed as mean ± SD. BW: body weight; Prec.: preconditioning; FA: filtered air