Table 1 Primers used for qRT-PCR
From: Critical roles of microRNA-141-3p and CHD8 in hypoxia/reoxygenation-induced cardiomyocyte apoptosis
Gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) | NCBI accession ID | Length of the amplicons |
|---|---|---|---|---|
miR-141-3p | The primer sequences are proprietary information of the company. (RiboBio Co., Ltd, Guangzhou, China) | |||
U6 | ATTGGAACGATA CAGAGAAGATT | GGAACGCTT CACGAATTTG | K00784 | 70Â bp |
CHD8 | CCTCACGCAC TGCTTCACCATC | CTCCTAGCCACC ACCTCATCCTC | NM_001347661 | 133Â bp |
GAPDH | GGTGGACCTCA TGGCCTACA | CTCTCTTGCTCTC AGTATCCTTGCT | NM_017008.4 | 84Â bp |