Fig. 4
From: Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier
a Surface antigen evaluation by flow cytometry. The human amniotic fluid stem cells (hAFSCs) were positive for CD29, CD44, CD90, and CD105; and negative for CD14, CD31, CD34, CD45, and CD117. IgG1-fluorescein isothiocyanate (FITC) and IgG2-phycoerythrin (PE) antibodies were used as isotype controls [30]. b Stem cell gene expression. The expression of stem cell genes Octa-4 and Rex-1 [31]