Fig. 3

HQP1351 regulates KIT oncogenic signaling proteins in vitro. Effect of HQP1351 (1351), ponatinib (pona) and imatinib (imat) on KIT and its downstream signaling proteins in GIST T1 (a) and GIST 430 (b) cells. After the cells were treated with the indicated concentrations of HQP1351, ponatinib, or imatinib for 24 and 72 h, cell lysates were collected for Western blot analysis. Note that tenfold higher concentrations of imatinib were used in imatinib-resistant cell lines GIST 430 to order to achieve the comparable results. p, phosphorylation; C, control