Table 1 Primer sequence for RT-qPCR
Genes | Primer sequences |
|---|---|
ANP | F: 5′-GGGGGTAGGATTGACAGGAT-3′ |
R: 5′-ACACACCACAAGGGCTTAGG-3′ | |
β-MHC | F: 5′-CTACAGGCCTGGGCTTACC -3′ |
R: 5′-TCTCCTTCTCAGACTTCCGC -3′ | |
TIMP2 | F: 5′-CCCGTAAGAAGGCTGACAGA-3′ |
R: 5′-CCCTCCAGACCCACTACCAT -3′ | |
Collagen I | F: 5′-ACAGGCGAACAAGGTGACAGAG -3′ |
R: 5′-GCCAGGAGAACCAGCAGAGC-3′ | |
Collage III | F: 5′-AGATGCTGGTGCTGAGAAGAAAC-3′ |
R: 5′-GCTGGAAAGAAGTCTGAGGAAGG-3′ | |
TGF-β | F: 5′-GGAAAGTGTTTCACCGCCAC-3′ |
R: 5′-ACTGACACGTGACACTGGAC-3′ | |
GAPDH | F: 5′-TTAGCACCCCTGGCCAAGG-3′ |
R: 5′-CTTACTCCTTGGAGGCCATG-3′ |