Fig. 2

LCZ696 alleviates Ang II-induced cardiac hypertrophy and fibrosis. Extracted cardiomyocytes were treated with Ang II (100 nM) alone or with LCZ696 (1.0 μM), or without any treatment. a Cardiac hypertrophy condition detected by 3[H]-leucine incorporation methodology. b mRNA expression of ANP, βMHC and TIMP2 in cardiomyocytes detected by RT-qPCR. c Size of cardiomyocytes detected by a-actinin immunofluorescence staining and percentage of cardiomyocyte size (scale bar = 25 μm). d Cardiac fibrosis assessed by 3[H]-proline incorporation methodology. e mRNA expression of collagen I, collagen III and TGF-β detected by RT-qPCR. F, Western blot analysis of ACE2 protein in cardiomyocytes. *p < 0.05 vs. cardiomyocytes without treatment; ^#p < 0.05 vs. cardiomyocytes treated with Ang II. Measurement data (mean ± S.D.) among multiple groups was analyzed by one-way ANOVA, followed by Tukey post hoc test. The experiment was conducted 3 times independently