Fig. 5
From: Babao Dan attenuates acute ethanol-induced liver injury via Nrf2 activation and autophagy
BBD-serum activates Nrf2 in cell culture model. BBD-serum up-regulates Nrf2 and its transcriptional targets HO-1 and GCLC in AML-12 cells. The cells were treated with 10% normal serum or 10% BBD-serum for 24 h and expression of Nrf2, HO-1 and GCLC were measured by a western blotting and b Real-time PCR. c Influences of Nrf2 knockdown on the protective effect of BBD-serum against ethanol-induced cell death. d Influences of HO-1 inhibition by its inhibitor SnMP on the protective effect of BBD-serum against ethanol-induced cell death. Values presented are from three replicates as mean ± SD. #P < 0.05 compared to control; *P < 0.05, **P < 0.01. EtOH 400 mM ethanol