Fig. 3

Loss of Tmem108 decreased DG adult neurogenesis. Representative images of BrdU assay, the low panel was from the rectangular area of the upper panel in a (wild type mice n = 5) or c (Tmem108 −/− mice n = 5); 2-month mice were injected ip with 300 mg/kg BrdU 24 h before sacrifice; incorporated BrdU was detected in sections by IHC using an anti-BrdU antibody (green), nuclei were counterstained with DAPI (blue). Quantification of BrdU positive cell number per mm DG inner edge (b) and per square mm DG region (d). Representative images of anti-Ki67 staining (red) from 2-month mice, the low panel was from the rectangular area of upper panel in e (wild type mice n = 4) or g (Tmem108 −/− mice n = 4); nuclei were counterstained with DAPI (blue). Quantification of Ki67 positive cell number per mm DG inner edge (f) and per square mm DG region (h) (**p < 0.01, ***p < 0.001)