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Fig. 1 | Cell & Bioscience

Fig. 1

From: 1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) attenuates gemcitabine-induced neutrophil extravasation

Fig. 1

PLAG inhibits gemcitabine-induced neutrophil extravasation into the peritoneum by down-regulating the expression of adhesion molecules in normal BALB/c mice. a Male BALB/c mice of 6–8 weeks of age were orally administrated with 50 or 250 mg/kg of PLAG, and then were intraperitoneally injected with 50 mg/kg gemcitabine. After 24 h, blood samples were collected by retro-orbital bleeding, and the number of blood neutrophils were determined by CBC analysis. Each group contains seven mice, and bars represent the mean ± SD. b The number of neutrophils from the blood and the peritoneal cavity was compared. Each group contains five mice. c, d The population of blood neutrophils was analyzed by flow cytometry. Red cell-lysed whole blood was stained with FITC-conjugated anti-Ly6G and PE-Cy7-conjugated anti-CD11b antibodies to determine the circulating neutrophil population. Ly6G+/CD11b+ cells were further stained with e APC-conjugated anti-L-selectin and f APC-conjugated anti-LFA-1 antibodies and were analyzed by flow cytometry to determine the expression of adhesion molecules. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001

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