Fig. 4

TIPE2 suppressed the proliferation and invasion of GIST cells by inhibiting Rac1. a After co-transfected with Rac1-specific siRNA and TIPE2 overexpression plasmid, GIST-T1 cells were applied to CCK8 assays. b After treatment with NSC23766, GIST-T1 cells were transfected with the TIPE2-overexpressing plasmid, and cell viability was measured by CCK8 assays. c GIST-T1 cells that transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids were used for CCK8 assays. d GIST-T1 cells that co-transfected with Rac1-specific siRNA and TIPE2 overexpression plasmid were applied to Transwell invasion assays. e After treatment with NSC23766, GIST-T1 cells were transfected with the TIPE2-overexpressing plasmid, and cell invasiveness was measured by Transwell invasion assays. F. GIST-T1 cells that transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids were used for Transwell invasion assays. **P < 0.01