Fig. 5

(+)-JQ1 reduces LPS induced neuroinflammation in vivo. a IL-1β-luc transgenic mice were intraperitoneally injected with LPS (2 μg in 2 μl), together with or without ICV injection of (+)-JQ1 (50 mg/kg). 4–6 h later, the in vivo fluorescence images were taken on and the fluorescence intensity was measured (n = 3). b, c Mice were ICV injected with LPS (2 μg in 2 μl), together with or without (+)-JQ1 (50 mg/kg). 4 h later, mice were sacrificed and brain tissue samples were collected. Reactive astrocytes (GFAP +) and microglia (Iba-1 +) were detected by IHC from brain tissue sections (scale bar: 100 μm) (b). GFAP (astrocyte) and CD68 (microglia) expression level were detected by immnoblot from brain tissue protein sample. immunoblot quantification was carried out by data normalization to β-actin level (c). Transcription levels of IL-1β, IL-6 and TNFα in the brain tissue were quantitatively analyzed by qRT-PCR. The data are presented as the mean ± SEM (n = 6, * p ≤ 0.05)