From: Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing
Effectora | Cas9 | Cas12a | Cas13a | Cas13b | Cas13d |
---|---|---|---|---|---|
Subtype | II-A | V-A | VI-A | VI-B | VI-D |
Target | dsDNA | dsDNA | ssRNA | ssRNA | ssRNA |
Nuclease domainb | RuvC-NTS, HNH-TS | RuvC-NTS, TS Nuc | 2 HEPN | 2 HEPN | 2 HEPN |
Pre-crRNA processing | RNase III | WED III | Helical 1 or HEPN2 | ? | ? |
tracrRNA | Yes | No | No | No | No |
Cut nature | Staggered, 5′ 1- or 3-nt overhangs | Staggered, 5 nt overhangs collateral activity-ssDNA | Collateral activity-ssRNA | Collateral activity-ssRNA | Collateral activity-ssRNA |
PAM/PFSc | 3′G-rich PAM | 5′T-rich PAM | 3′ PFS: H | 5′PFS: D;3′ PFS:NAN or NNA | No? |
Median size | 1228 aa | 1268 aa | 1228 aa | 1128 aa | 928 aa |