Fig. 3

CCDC47 is localized in the endoplasmic reticulum (ER) and regulates calcium flux in rat H9C2 cardiomycotyes. a Schematic of CCDC47 as a transmembrane protein that contains an N-terminal ER signaling peptide and calcium binding domain. b Immunohistochemical staining of CCDC47 revealed co-localization with the ER marker SERCA2 in H9C2 myocytes. Green channel indicates CCDC47, red channel indicates SERCA2, and merged image indicates co-localization in yellow. Nuclear staining (blue) was performed with DAPI. c Cytofluorogram depicts degree of overlay between CCDC47 and mitochondria labeled with mito-tracker (left panel) and overlay between CCDC47 and SERCA2 (right panel). d Quantification of degree of overlay using Pearson’s coefficient, M1, and M2 values. All three measurements revealed significant co-localization of CCDC47 with SERC2A compared to mito-tracker. Bar graphs indicate mean + standard error of n = 3–4 independent biological replicates. *p < 0.05 and ***p < 0.001 compared to mito-tracker