Fig. 5
From: The development of salt-sensitive hypertension regulated by PSGL-1 gene in mice
The number of PBMC adhering to Bend3 cells co-cultured in normal and high salt medium. The Bend3 cells were co-cultured with PBMC from PSGL-1+/+ and PSGL-1−/− mice stained with the ratio-fluorometric 2′,7′-bis-(2-carboxyethyl)-5-carboxyfluorescein acetoxymethyl ester (BCEC-F-AM) at normal salt (133 mM) and high salt (173 mM) medium. Fluorescent signals were captured using a confocal microscope (Leica). Green represents the number of adhered PBMC