Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells

Cell & Bioscience

Table 1 Efficiency of HDR and mutation correction in HCT-116 cells transfected with a Cas9 co-expression vector in the presence or absence of ssODN and SCR7

Donor	Groups	Rate of HDR (%)	Correction (%)
No donors	Cas9	4/107 (3.7)	3/107 (2.8)
No donors	Cas9 + SCR7	8/114 (7.0)	7/114 (6.1)
β-cateninWT-96	Cas9 + ssODN	13/155 (8.4)	11/155 (7.1)
β-cateninWT-96	Cas9 + ssODN + SCR7	22/151 (14.6)*	21/151 (13.9)*

HCT-116 cells were transfected with the pCS2-Cas9-IRES-eGFP-β-catenin-gRNA(ΔTCT) co-expression vector with or without the addition of β-cateninWT-96 ssODN and SCR7 (10 μM) treatment. The rates of HDR and mutation-correction were derived from direct DNA sequencing of GFP-positive cells as described in the methods. These statistical assays were performed by One-side Chi square test
* p < 0.05 compared to the corresponding Cas9 + ssODN group

ISSN: 2045-3701