Fig. 5
From: The lncRNA MEG3 downregulation leads to osteoarthritis progression via miR-16/SMAD7 axis
MiR-16 reversed MEG3-mediated anti-proliferation and pro-apoptosis in IL-1β-induced chondrocytes. IL-1β-induced chondrocytes were transfected with si-NC, si-MEG3, si-MEG3 + anti-miR-NC, si-MEG3 + anti-miR-16, pcDNA-NC, MEG3, MEG3 + miR-NC or MEG3 + miR-16 mimics. a Cell viability of treated IL-1β-induced chondrocytes was determined at OD450 nm by Cell Counting Kit-8. b Cell apoptosis of treated IL-1β-induced chondrocytes was assessed by flow cytometry using Annexin V-FITC Apoptosis Assay kit. c Bax and Bcl2 expression were detected in treated IL-1β-induced chondrocytes by western blot analysis. *P < 0.05 vs. negative control