Fig. 3

MEG3 was involved in miR-16 pathway. a Sequence alignment of miR-16 and the putative binding sites within the wild-type MEG3, and mutation in the MEG3. b The interaction between MEG3 and miR-16 was detected by RNA immunoprecipitation (RIP) with Ago2 antibody. c The luciferase activity was detected in HEK 293T cells transfected with MEG3 (WT) or MEG3 (MUT) reporter vector together with miR-16 mimics or anti-miR-16. d The correlation between MEG3 and miR-16 expression in cartilage tissues of rat OA model by RT-qPCR. GAPDH and U6 were used as internal reference. e The relative expression of miR-16 was examined in IL-1β-induced chondrocytes transfected with pcDNA-MEG3, si-MEG3 or control. *P < 0.05 vs. negative control