Fig. 1

Down-regulation of Gadd45β expression in liver cancer tissues and liver cancer cell lines. a The typical IHC images from tumor and peri-tumor tissues with Gadd45β staining were obtained. The tumor tissues showed negative staining. The peri-tumor tissues indicated the obviously positive staining. b The IHC staining of 100 paired tissue samples was classified. The peri-tumor tissues showed the 80% positive staining; the tumor tissues indicated only 20% positive staining. c The Gadd45β mRNA expression in 20 paired of fresh tissue samples was examined by RT-PCR assay. The tumor tissues showed the relatively lower Gadd45β expression, compared to peri-tumor tissues. GAPDH was used as an internal reference. (**P < 0.01; ***P < 0.001). d Western blot analysis showed the lower Gadd45β protein level in tumor tissues, compared to peri-tumor tissues. The protein levels were normalized by a comparison with the GAPDH level. e, f Gadd45β expression in HCC cell lines (SMMC-7721, Hep-3B) and normal liver cells (L-02) was examined by PCR (e) and western blot (f), GAPDH was used as an internal reference