Fig. 1

Regulation of IFNα, IFNβ and IFNε by various cytokines in A2EN cells and primary cervical cells. A2EN or primary cervical epithelial cells were treated with stated cytokines at 1 or 10 ng/ml for 4 or 24 h. Total RNAs were prepared and gene expression of IFN⍺, IFNβ and IFNε was measured by real-time RT-PCR. Induction of each gene (as fold-change relative to untreated control) was calculated using the ΔΔCT method as described in “Materials and methods”. The value of 1 indicates the level of gene expression of untreated control cells. Data for TNFα and IL-1β are mean ± SD of four independent experiments in A2EN cells (a) and of experiments from primary cervical cells from different donors (b). Average of fold-changes of other cytokines-treated samples relative to untreated samples from four different experiments were summarized in c. Numbers in bold represents significant changes (*p < 0.05). ⍭p=0.057–0.07