Fig. 3

The effect of sirtinol on the proliferation and differentiation of T cells subset. a The recipient spleens were harvested 7 days after transplantation. CD3⁺ T cells, CD4⁺ T cells and CD8⁺ T cells from splenocyte were measured by flow cytometry. b The recipient spleens were harvested 7 days after transplantation. CD4⁺ T cells were isolated and CD25⁺ and Foxp3⁺ T cells were measured by flow cytometry. Shown are representative of five separate experiments. c The recipient spleens were harvested 7 days after transplantation. CD4⁺ T cells were isolated and IL-17A⁺ T cells were measured by flow cytometry. Shown are representative of five experiments. d Percentages of CD4⁺, CD25⁺Foxp3⁺ and IL-17A⁺ cells were presented. e ELISA assays were performed to test the IL-17A levels on day 7 after transplantation. The data are expressed as mean ± SD (n = 5). *P < 0.05, **P < 0.01, compared with DMSO group