Fig. 6

Immunostaining of Iba1 shows blunted recruitment of Iba1+ microglial cells in CD1 mice relative to C57BL/6 mice after various length of cuprizone exposure. a Representative images of Iba1+ microglia at the midline of the corpus callosum. Scale bar, 30 μm. b Quantification of cell density counts. CD1 mice and C57BL/6 mice show comparable numbers of Iba1 and to-pro-3 double positive microglia under control conditions, and significant differences between strains were observed at 4, 6 and 7 weeks of treatment (asterisks above the brackets indicate significant interaction between strain and cuprizone in two way ANOVA analysis, P < 0.01). Asterisks above the bars indicate significant difference compared to respective controls (t-test, *P < 0.05; **P < 0.01). Error bars show SEM. N numbers are indicated on individual bars