Fig. 3

Down-regulation of HK2 inhibits viability and glycolysis and induces apoptosis in LSCC cells. a Western blot analysis was performed to detect the protein level of HK2 in AMC-HN-8 and TU212 cells transfected with si-HK2 or treated with an inhibitor of HK2 (3-BrPA). b The cell viability of AMC-HN-8 and TU212 cells was determined by CCK-8 assays. c, d Glucose consumption and lactate production in AMC-HN-8 and TU212 cells. e, The cell apoptosis of AMC-HN-8 and TU212 cells was determined by flow cytometry analysis. *P < 0.05. NC represents negative control