Condition of column | Initial protein (µg ± SD)a | Flow-through (µg ± SD)a | Bound protein (µg ± SD)a | % of bound proteinb |
---|---|---|---|---|
Column set up | ||||
Recombinant LC3 binding control | 50 | 12.67 ± 0.54 | 37.33 ± 0.54 | 74.65 ± 0.96 |
Empty pET17b competent binding control | 50 | 25.58 ± 0.27 | 24.42 ± 0.27 | 49.24 ± 0.01 |
Bacterial protein (0.5 µg/µl) applying step | ||||
B. pseudomallei WT | 36.54 ± 0.54 | 23.08 ± 1.63 | 13.46 ± 1.09 | 36.87 ± 3.53 |
B. pseudomallei rpoS mutant | 37.12 ± 1.36 | 24.42 ± 0.27 | 12.69 ± 1.63 | 34.14 ± 3.15 |
Negative controlc | 24.42 ± 0.27 | 19.42 ± 1.36 | 5.00 ± 1.09 | 20.50 ± 4.68 |