Fig. 4

The requirement for UTX and JMJD3 in NKT cell development is cell intrinsic. Thymocytes were isolated from chimeric mice that were reconstituted with bone marrow from WT or UTX/JMJD3 DKO mice mixed with WT CD45.1 congenic bone marrow 5–7 weeks prior to analysis. a Thymocytes were stained with CD1d tetramer reagent and other markers of NKT precursors. Plots were gated by light scatter on lymphocytes, and dead cells were excluded by DAPI staining. Shown are CD45.2⁺tetramer⁺ cells. b Representative plots of donor and host markers are shown for each thymic NKT population P1–P3. c Quantification of results from 3 experiments with 10 mice in the WT control and 9 mice in the DKO experimental group. In order to control for reconstitution efficiency, the fraction of the donor population was normalized to the fraction of donor cells in splenic B cells. This serves as a control, because these cells do not express CD4-cre and do not excise the floxed alleles. Asterisk indicates p < 0.005 by two-tailed T test. d As in c, the CD45.2 contribution to splenic CD4 or CD8 T cells was assessed by FACS. The difference between WT and DKO is significant (p < 0.005). Any difference in CD8 reconstitution is not significant