Hepatitis B virus promotes cancer cell migration by downregulating miR-340-5p expression to induce STAT3 overexpression

Table 1 Primers used in this study

Gene	Primer sequences (5′–3′) forward (F), reverse (R)	GenBank accession number	Product length(bp)
GAPDH	F: GAGAAGGCTGGGGCTCATTT	NM_001289745	156
GAPDH	R: TAAGCAGTTGGTGGTGCAGG	NM_001289745	156
STAT3	F: GAGGACTGAGCATCGAGCA	NM_139276	85
STAT3	R: CATGTGATCTGACACCCTGAA	NM_139276	85
STAT3-3′UTR	F: ATCCGCTCGAGATGGCCCAATGGAATC	NM_139276	2435
STAT3-3′UTR	R: CGACGCGTGAGGTCAACTCCATGTCAAAG	NM_139276	2435
STAT3-3′UTR-WT1+WT2	F: GCGCGCGCGGACTAGTTTTATAAATAGACTTATTTTCCT	NM_139276	1191
STAT3-3′UTR-WT1+WT2	R: CGACGCGTTTATAAACCACCTTATAGGTAGGTAAGC	NM_139276	1191
STAT3-3′UTR-MUT1	F: GCGCGCGCGGACTAGTT CCGAT AATAGACTTATTTTCCT	NM_139276	1191
STAT3-3′UTR-MUT1	R: CGACGCGTTTATAAACCACCTTATAGGTAGGTAAGC	NM_139276	1191
STAT3-3′UTR-MUT2	F: GCGCGCGCGGACTAGTTTTATAAATAGACTTATTTTCCT	NM_139276	1191
STAT3-3′UTR-MUT2	R: CGACGCGTAATAT AACCACCTTATAGGTAGGTAAGC	NM_139276	1191
STAT3-3′UTR-MUT1+MUT2	F: GCGCGCGCGGACTAGTT CCGAT AATAGACTTATTTTCCT	NM_139276	1191
STAT3-3′UTR-MUT1+MUT2	R: CGACGCGTAATAT AACCACCTTATAGGTAGGTAAGC	NM_139276	1191

ISSN: 2045-3701