Fig. 4

Decreased Otg1 expression leads to impaired hormone secretion. a H&E staining of pancreas from P11 Otg1 ^PB/PB and wild-type littermates. b Relative pancreas islet cell sizes of P11 Otg1 ^PB/PB (n = 31) and wild-type (n = 20) littermates. c Scanning EM of islet cells of P6 Otg1 ^PB/PB and wild-type littermates. White arrows indicate insulin granules. N: nucleus. G: Golgi. d Insulin levels of glucose stimulated insulin secretion (GSIS) after isolation of islets isolated from Otg1 ^PB/PB (n = 7) and wild-type (n = 8) littermates at P1. e Western blot showed the decreased Otg1 expression in GH3 cells transfected with small hairpin RNAs, but not scramble shRNAs. GAPHD serves as the loading control. f Released growth hormone levels in Otg1 knockdown cells compared with cells transfected with scramble shRNAs, which is arbitrary set as 1. g Growth hormone levels in Otg1 knockdown cells compared with cells transfected with scramble shRNAs, which is arbitrary set as 1. *p < 0.05, **p < 0.01, ***p < 0.005